Erratum: Eukaryotic ribosome display with in situ DNA recovery
نویسندگان
چکیده
منابع مشابه
Interaction of tRNA with Eukaryotic Ribosome
This paper is a review of currently available data concerning interactions of tRNAs with the eukaryotic ribosome at various stages of translation. These data include the results obtained by means of cryo-electron microscopy and X-ray crystallography applied to various model ribosomal complexes, site-directed cross-linking with the use of tRNA derivatives bearing chemically or photochemically re...
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Ribosome display is an in vitro evolution technology for proteins. It is based on in vitro translation, but prevents the newly synthesized protein and the mRNA encoding it from leaving the ribosome. It thereby couples phenotype and genotype. Since no cells need to be transformed, very large libraries can be used directly in selections, and the in vitro amplification provides a very convenient i...
متن کاملThe Eukaryotic Ribosome MOLECULAR BIOLOGY
The past decade has seen a remarkable advance in our understanding of ribosomes, the large protein-RNA machines in all cells that use genetic information to synthesize proteins in a process known as translation. Ribosomes from all species consist of two subunits: a small subunit that decodes messenger RNA (mRNA), and a large subunit that catalyzes peptide bond formation between the growing poly...
متن کاملSelection of Anti-Sulfadimidine Specific ScFvs from a Hybridoma Cell by Eukaryotic Ribosome Display
BACKGROUND Ribosome display technology has provided an alternative platform technology for the development of novel low-cost antibody based on evaluating antibiotics derived residues in food matrixes. METHODOLOGY/PRINCIPAL FINDINGS In our current studies, the single chain variable fragments (scFvs) were selected from hybridoma cell lines against sulfadimidine (SM(2)) by using a ribosome libra...
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Single chain variable fragments (scFvs) against citrinin (CIT) were selected from a scFv library constructed from the splenocytes of non-immunized mice by an improved eukaryotic ribosome display technology in this study. Bovine serum albumin (BSA)/ CIT-BSA and ovalbumin (OVA)/ CIT-OVA were used as the antigens to select specific anti-CIT scFvs. Eukaryotic in situ RT-PCR method was used to recov...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2007
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0907-763